IPA is also a disinfectant its use in World Health Organization-recommended sanitizer formulations against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 5 led to supply shortages and surging prices during the Coronavirus Disease 2019 (COVID-19) pandemic. Additionally, acetone has potential in the fuel sector to reduce emissions and enhance the performance of existing fuels 1, 2 and as a precursor for green diesel or jet fuel substitutes 3, 4. Both molecules are industrial solvents as well as platform chemicals for the production of materials such as acrylic glass (polymethyl methacrylate) and polypropylene. Acetone and isopropanol (IPA) are two such examples, with a combined global market of more than US$10 billion. Most commodity chemicals are derived exclusively from fresh fossil resources, such as oil, natural gas and coal. The accelerating climate crisis has intensified demand for environmentally sustainable, carbon-negative manufacturing of chemicals and materials. We expect that our approach can be readily adapted to a wide range of commodity chemicals. These results show that engineered acetogens enable sustainable, high-efficiency, high-selectivity chemicals production. Unlike traditional production processes, which result in release of greenhouse gases, our process fixes carbon. Life cycle analysis confirmed a negative carbon footprint for the products. Finally, we scaled-up our optimized strains for continuous production at rates of up to ~3 g/L/h and ~90% selectivity. Next, we used omics analysis, kinetic modeling and cell-free prototyping to optimize flux. Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum. Here we describe the development of a carbon-negative fermentation route to producing the industrially important chemicals acetone and isopropanol from abundant, low-cost waste gas feedstocks, such as industrial emissions and syngas. Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation.
Nature Biotechnology volume 40, pages 335–344 ( 2022) Cite this article Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale
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